Composite

Part:BBa_K1747012:Design

Designed by: Harry Bennett   Group: iGEM15_LaVerne-Leos   (2015-08-26)


Fatty acid promoter - GFP - crt Z,E,B,I,Y - term - Repressor circuit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3347
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2883
    Illegal NgoMIV site found at 3013
    Illegal AgeI site found at 2098
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734


Design Notes

There is no scar sequence in between the first fatty acid promoter and the following RBS


Source

All sequences were acquired from iGEM

References